Wojciech Debek(1), Lech Chyczewski(2), Karl Juergen Oldhafer(3)

BN52021 Inhibits Activation of Peritoneal Mast Cells Caused by Hemorrhage and Blood Volume Restoration *

1)Department of Pediatric Surgery,
2)Department of Pathological Anatomy, Bialystok, Poland;
3)Department of Abdominal Surgery and Transplantation, Hanover Medical School, Germany

Abstract

An extensive blood loss activates generalized inflammatory response. Abdominal organs and especially intestines are very sensitive to the ischemia-reperfusion in- sults due to hemorrhagic shock (HS) and blood volume restoration. Previously ob- tained results suggest that studies on peritoneal lavage fluid (PLF) can contribute to elucidation of inflammatory processes in abdominal organs in HS. Histamine (H) levels, total cell, and mast cell (MC) numbers, and MC ultrastructure in the fluid lavaged from peritoneal cavity were compared in the following groups of rats: control (gr. 1), sham operation (gr. 2), untreated hemorrhagic shock (gr. 3), shock treated with blood volume restoration with lactated Ringerís solution (LR) (gr. 4), shock treated with platelet activating factor (PAF) - receptor antagonist Ginkgolide B (BN52021), and LR (gr. 5). A shock-related significant increase in to- tal cell numbers, MC numbers, MC degranulation, and histamine levels in PLF were observed. The restoration of blood volume caused further elevation of the above phenomena (gr. 4) while BN52021 seemed to inhibit peritoneal MC mobili- zation and degranulation as well as to attenuate increase in peritoneal H level (gr. 5). The peritoneal cavity is a place of rapid and strong reaction to hemorrhage. Evaluation of peritoneal histamine levels might be helpful in the monitoring of shock dependent intra-abdominal processes. Peritoneal MC mobilization and de- granulation, and increase in histamine level is inhibited by BN52021.

Address for correspondence and reprint requests to:
W. Debek M. D.,
Gajowa 81/33, 15-794 Bialystok,
e-mail: debek_w@2n.pl

*This study was supported by the Foundation of the Polish-German Cooperation. Project No1262/94.