Alina Grzanka

The Expression of Vimentin in HL-60 Cells Induced with Etoposide Using Immunofluorescence and Immunogold Methods

Department of Clinical Pathomorphology, University School of Medical Sciences, Bydgoszcz Institute of Biology and Environment Protection, Bydgoszcz University of Kazimierz Wielki

Abstract

HL-60 leukemic cells were treated with 6 different doses of etoposide for 72 hours. Changes in the distribution of vimentin were found to be dependent on the concentration of etoposide. As compared with control cells there were distinct changes in cells incubated with 100 and 200µM/L of the drug. The size of cells treated with 100µM/L and especially with 200µM/L increased, but the number of cells decreased. In control cells and those treated with 0.02, 0.2 and 2µM/L etoposide, vimentin was seen rather as a ring often with the increased concentration near one pole of the cells. Cells at 20µM/L etoposide showed the same staining pattern but more brighter cells were found. The addition of 100µM/L and 200µM/L etoposide to cells resulted in diffusely distributed fluorescence staining, which often appeared as a quite dense network around the nucleus. Immunogold labelling was observed in cells treated with all doses of etoposide and control cells. Labelling was localized in the nucleus but also in the cytoplasm but rather in the area of the nucleus.